A mouse lymphoma cell mutant whose major protein product is ornithine decarboxylase.
نویسندگان
چکیده
منابع مشابه
Expression of an ornithine decarboxylase dominant-negative mutant reverses eukaryotic initiation factor 4E-induced cell transformation.
pMV7-4E cells (4E-P2), which overexpress translation initiation factor eIF-4E, contain elevated levels of ornithine decarboxylase (ODC), the first and rate-limiting enzyme in polyamine biosynthesis. We have shown previously that this induction appears to be related to the transformed phenotype of these cells (L. M. Shantz and A. E. Pegg, Cancer Res., 54: 2313-2316, 1994). To test whether increa...
متن کاملStrain polymorphism and tentative mapping of mouse ornithine decarboxylase.
SOURCE/DESCRIPTION The plasmld p0D45~contains a 1.6 kb of a omithine decarboxyiase cDNA . POLYMORPHISM Constant Eco RI bands vere found at 3.6, 4.4, 5.5, 6.9, 8.5, 11.5, 13.5 and 19 kb. Variant bands vere found at 4.7 and 6.2 kb. FREQUENCY The 4.7 band is present in BALB/c, not in C57BL/6, DBA/2 and C3Hf, and segregate in CxB recombinant inbred (RI) strains. The 6.2 kb band is present in DBA/2 ...
متن کاملMouse ornithine decarboxylase gene: cloning, structure, and expression.
We used molecular cloning to isolate a functional gene for mouse ornithine decarboxylase (OrnDCase; L-ornithine carboxy-lyase, EC 4.1.1.17) from a cell line in which that gene had been selectively amplified. The position of the 5' terminus of the mRNA was identified, and the coding sequence was shown to be preceded by a 312- or 313-nucleotide (nt) untranslated leader. The latter is highly G + C...
متن کاملAndrogen-regulated ornithine decarboxylase mRNAs of mouse kidney.
Ornithine decarboxylase, the first enzyme of the polyamine biosynthetic pathway, is induced by androgens in the mouse kidney. We have isolated from a kidney cDNA clone bank two plasmids, pODC1440 and pODC934 , that contain different cDNA inserts corresponding to ornithine decarboxylase mRNA. Identification was based upon the ability of plasmid-specific mRNAs to encode a 53,000-dalton polypeptid...
متن کاملMolecular cloning and expression of the mouse ornithine decarboxylase gene.
We used mRNA from a mutant S49 mouse lymphoma cell line that produces ornithine decarboxylase (OrnDCase) as its major protein product to synthesize and clone cDNA. Plasmids containing OrnDCase cDNA were identified by hybrid selection of OrnDCase mRNA and in vitro translation. The two of these with the largest inserts together span 2.05 kilobases of cDNA. Southern blot analysis of DNA from wild-...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1983
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)44131-7